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KMID : 0624620190520100619
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BMB Reports
2019 Volume.52 No. 10 p.619 ~ p.624
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Deficiency of calpain-6 inhibits primary ciliogenesis
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Kim Bo-Hye
Kim Do-Yeon
Oh Su-Min
Ko Je-Yeong
Rah Gyu-Yeong
Yoo Kyung-Hyun
Park Jong-Hoon
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Abstract
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The primary cilium is a microtubule-based structure projecting from a cell. Although the primary cilium shows no motility, it can recognize environmental stimuli. Thus, ciliary defects cause severe abnormalities called ciliopathies. Ciliogenesis is a very complex process and involves a myriad of components and regulators. In order to excavate the novel positive regulators of ciliogenesis, we performed mRNA microarray using starved NIH/3T3 cells. We selected 62 murine genes with corresponding human orthologs, with significantly upregulated expression at 24 h after serum withdrawal. Finally, calpain-6 was selected as a positive regulator of ciliogenesis. We found that calpain-6 deficiency reduced the percentage of ciliated cells and impaired sonic hedgehog signaling. It has been speculated that this defect might be associated with decreased levels of α-tubulin acetylation at lysine 40. This is the first study to report a novel role of calpain-6 in the formation of primary cilia.
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KEYWORD
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Acetylated α-tubulin, Calpain-6, mRNA microarray, Primary cilia, Tubulin code
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